Yurchenko P. Role of hydrogen sulfide in the mechanisms of brain damage in hyperhomocysteinemia.

Object - condition H2S system in the brain with hyperhomocysteinemia (HHC); goal – establish the role of the hydrogen sulfide system in the mechanisms of brain damage in conditions of the experimental hyperhomocysteinemia and substantiate the approaches to its correction; methods – biochemical, ELISA, pathophysiological, pharmacological, morphological, statistical; results – there was defined the role of H2S system in the mechanisms of the formation of a favorable metabolic pattern in the brain of rats under the condition of isolated and combined HHC and experimentally based approach to correction of neurodegenerative processes in disorders of homocysteine metabolism using H2S donor, vitamin and polymicroelements funds; for the first time HHC was shown to cause a decrease in H2S content in the brain, been associated with a reduction in activity of piridoksalfosfat-dependent H2S-synthesizing enzymes, and piridoksalfosfat-independent tiosulfatditiolsulfidtransferazy, increasing the overall utilization rate of H2S to sulfite reduction of activity in the brain tissue; the H2S deficiency was proved to be an independent factor, potentiating the development of oxida-tive stress and inhibition of the synthesis of glutathione, deepening the imbalance in the exchange of adenine nucleotides and the energy deficit in the brain in HHC; for the first time an inhibitor of synthesis of H2S, aminooxyacetate was revealed to potentiate the incensement of neurotoxic effect HHC, and an inorganic donor of H2S (NaHS) was shown to accelerate the elimination of excess homocysteine, to promote the normalization of metabolic pattern in the brain, to reduce deprimiruty effects of HHC on orientate and research activity and emotional and motivational sphere of animals; branch – medicine.