Grynevych N. The system of sanitary-hygienic measures in recirculating aquatic systems of industrial fish farms

Українська версія

Thesis for the degree of Doctor of Science (DSc)

State registration number

0518U000693

Applicant for

Specialization

  • 16.00.06 - Гігієна тварин та ветеринарна санітарія

06-07-2018

Specialized Academic Board

Д 55.859.04

Sumy National Agrarian University

Essay

Seasonal fluctuations of hydrochemical and toxicological parameters of water in RAS and waste water have been established. It was first established that the amount of psychrotrophic microflora in water of RAS modules is on average 1,3 times higher than that of MAFAnM. Species Pseudomonas, Acinetobacter, Aeromonas, Alcaligenes and Flavobacterium are the dominant among the psychrotrophs (90%), which gives rise to consider them an autochthonous microflora of RAS. Оn the patterns of microflora colonization of different types of biofilter fillers during start and operation of the RAS for rainbow trout growing were obtained. The dynamics of the nitrification process in the biofilter reactor using different types of fillers was experimentally determined. The dependence of this process on the type of filler, the quantitative content of nitrifying and denitrifying microorganisms, and the density of the formed microbial biofilms on the filler was found. The idea of nitrite poisoning development in rainbow trout during the RAS start was expanded. The dynamics of changes in the hematological parameters, the histological structure of gill epithelium, liver and kidneys as well as the number of damaged chromosomes in gill cells were established. The expediency of using the developed biofilter microbiological starter “Filtronorm D” during RAS start or after implementation of treatment and prophylactic measures on infectious diseases for the rapid formation of active nitrifying and denitrifying microbiocenosis of biofilter has been theoretically substantiated and experimentally established. The theoretically and practically proved the necessity of using a method of water disinfecting in RAS by means of ultraviolet bactericidal lamp, which enables safe disinfection without disturbing the biofilter microbiocenosis. Positive influence of vitamin supplement “Hanaminovit” in young rainbow trout feeding thanks to immunity and growth rate increasing was revealed. The positive effect of this supplement on the larvae productivity when using it in feeding of the adult rainbow trout was also observed.

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